Fixing biological samples through microwave irradiation.
نویسندگان
چکیده
منابع مشابه
Pressurized Microwave Digestion Metal Determination of Biological Samples for
The utility of microwave oven for simple and rapid acid digestion of zoological and botanical materials was investigated by using a double Teflon vessel with a polypropylene jacket. The microwave-assisted digestion of samples with the HNO3-HCI-HCIO4-HF mixture in the bomb offers the advantage of taking much shorter process for sample preparation. The digestion was completed within 15 min even u...
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(1) "Standard Methods for the Examination of Water and Wastewater", 13th ed., American Public Health Association, Washington, DC, 1971, p 339. (2) "Methods for Chemical Analysis of Water and Wastes", U.S. Environmental Protection Agency, Washington, DC, 1974, p 157. (3) L. K . Wang, J. Am. Water Works Assoc., 67 (1). 19-21 (1975). (4) L. K . Wang, W. W. Shuster. and P. J. Panzardi, J. Am. Water...
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Microwave irradiation (MWI) has been applied to the development of rapid methods to process biological samples for scanning electron microscopy (SEM). In this paper we propose two simple and quick techniques for processing bacteria (Proteus mirabilis and Vibrio mimicus) for SEM using MWI. In the simplest methodology, the bacteria were placed on a cover-glass, air-dried, and submitted to conduct...
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Microwave-assisted sample preparation using diluted nitric acid solutions is an alternative procedure for digesting organic samples. The efficiency of this procedure depends on the chemical properties of the samples and in this work it was evaluated by the determination of crude protein amount, fat and original carbon. Soybeans grains, bovine blood, bovine muscle and bovine viscera were digeste...
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The recent advances in instrumentation for amino acid analysis and highperformance liquid chromatography (HPLC) have shortened the net analysis time for amino acid analysis to less than an hour for each individual sample. Therefore the rate-determining and most essential step of a successful amino acid analysis now lies in the skillful preparation of protein hydrolysates. The commonly used conv...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1990
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.28.535